FastQCFastQC Report
Mon 27 Feb 2023
SRR3128368.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128368.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1992987
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268161.3455180590741436No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38150.19142121850267965No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG33490.1680392295584467No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG32270.16191776464171617No Hit
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC30980.15544506813140277No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC22050.11063795197861301No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT84150.084.723531
AGTACGG2650.076.310021
TAGGGCA11450.075.5283054
AGGGCAT20700.071.748715
AGTAAGG11500.071.564751
AGTAGGG32900.071.2856452
ATAGGGC13950.070.752623
ATAGGGA17400.070.499923
TAGGGTA7450.068.765034
GAATAGG12800.068.705361
GTAGGGA17250.068.66083
AGGGTAA7950.068.5785455
ATAGCGG4900.068.1430051
GTAGGGC8450.067.857923
AGGGATG26250.067.6799245
TAGAGGG38900.067.66062
CGTAGGG6850.067.240812
AGATAGG12050.066.7372061
AAGAGGG69650.066.467992
ACGGGTC2700.066.148084
Produced by FastQC (version 0.11.5)