FastQCFastQC Report
Mon 27 Feb 2023
SRR3128367.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128367.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1699878
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237541.397394401245266No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33080.19460220086382668No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG29980.17636559800173895No Hit
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC28310.16654136355667876No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG26560.1562465071022744No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC18400.10824306214916599No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT77650.085.249991
AGTACGG2500.077.21371
TAGGGCA10250.077.122314
ATAGCGG3050.075.639171
AGGGATG23000.072.541055
CGTAGGG5900.071.7790762
ATAGGGC11550.071.701013
GTAGGGC7600.071.1998143
AGGGAAT17200.070.4976355
AGTAGGG27850.069.9491652
AGTAAGG10300.069.936631
AGGGCAT16450.069.711955
ATAGGGA14900.069.4753953
TAAGGGA14450.069.3595663
AGGGACT25700.069.308975
ACGGGTA1700.069.1967544
GTAGGGA15300.069.196753
TAGAGGG32450.068.878922
TAGGGTA6300.068.713474
TAGACGG2550.068.314361
Produced by FastQC (version 0.11.5)