FastQCFastQC Report
Mon 27 Feb 2023
SRR3128366.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128366.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1488395
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207691.3953957114878779No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAGCAAGCG77060.5177389066746395No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG50540.33956039895323487No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC47100.31644825466358056No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG44790.30092818102721386No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30810.20700150161751416No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA29540.19846882044081043No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATG18120.12174187631643482No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCAGCAAGCGTC17220.11569509438018805No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC16390.11011861770564937No Hit
AGAGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTT16210.10890926131840002No Hit
AAAGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTT15550.10447495456515239No Hit
ACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG15420.10360153050769452No Hit
CGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT15130.1016531229949039No Hit
AGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT14940.10037658014169626No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62600.086.128411
CCGTACA33200.077.004343
CGTACAC33050.076.500664
TCCGTAC34300.076.452992
ATCCGTA34550.076.392531
CGTAGGG3150.074.59572
TAGGGTA6150.074.1226654
AGGGCAT10950.073.819095
ATAGAGG8950.073.593781
AGGGTAA6400.072.6958545
GTACACC34950.072.207375
AGTAGGG21750.071.0871352
TACACCC33650.070.38826
TAGGGCA7700.070.187784
GAGGGTA7250.070.006794
AGGGAAT12300.069.919835
ATAGGGA10150.069.9141853
GTAGGGA7950.069.754033
AGGGTAC7300.069.527285
ACGGGTA1150.069.471314
Produced by FastQC (version 0.11.5)