FastQCFastQC Report
Mon 27 Feb 2023
SRR3128365.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128365.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1649400
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248971.509457984721717No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG56480.34242754941190734No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG49600.30071541166484783No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC49450.29980599005699043No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAGCAAGCG44290.26852188674669575No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT35540.21547229295501397No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA20880.12659148781375046No Hit
AGAGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTT17670.10712986540560203No Hit
AGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT17030.10324966654541046No Hit
CGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT16770.10167333575845762No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT79700.086.084651
CGTAGGG3350.084.185562
TAGGGTA6150.074.900064
GAATAGG8300.074.220171
AGTAGGG26600.072.272462
AGGGCAT14800.071.77365
AGGGTAC8200.071.6496665
TAGGGCA8450.071.200744
TAGTAGG7850.070.6872561
ACGGGTA1600.070.505414
ATAGGGA12100.070.311183
TATAGGG11700.069.90282
ATAGGGC8150.069.78453
GTAGGGA10100.069.341963
AGAGGGC21000.069.162453
ATAGAGG9800.068.61811
AAGGGTA10550.068.61194
TAAGGGA13550.067.9904563
AGGGTAT9250.067.581515
ATGAGGG18300.067.5516362