FastQCFastQC Report
Mon 27 Feb 2023
SRR3128364.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128364.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1398450
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219351.5685222925381672No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG47580.3402338303121313No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC42900.306768207658479No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG42050.30069004969787977No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30900.22095891880296042No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAGCAAGCG21640.15474275090278522No Hit
CGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT15360.10983588973506382No Hit
AGAGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTT14750.10547391755157495No Hit
AGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT14470.10347170081161286No Hit
AAAGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTT14110.10089742214594731No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA1000.089.4148944
CGTTTTT71650.086.586221
TAGGGTA5850.075.618534
TAAGGGA12700.075.59323
AGTACGG1500.075.312931
ATGCGGG6100.074.062382
CGTAGGG3200.073.531982
AGTAAGG6650.072.906311
ACGGGAT3600.071.897944
AGGGTAC7550.071.594365
AGAGGGC15250.071.5936363
AGTAGGG21900.071.342812
AGGGCAT11250.071.0271455
AGGGTAT7550.070.97185
TAGGGCA7450.070.748634
ATAGAGG8850.070.206971
AAGGGTA8050.070.152254
ATGAGGG18050.070.1344452
GTAGGGC5450.069.9430853
AGGGAAT14300.069.3544545