FastQCFastQC Report
Mon 27 Feb 2023
SRR3128362.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128362.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1595794
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205571.2881988527341248No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG43000.26945833860761476No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGCGTCTAA41900.2625652183176525No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC40460.2535414972107929No Hit
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG36800.23060620606419122No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA21580.13523048714307737No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT19510.12225888805196661No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74900.086.63041
CGTAGGG4700.077.999692
TAGGGCA8800.073.170164
AGTAGGG24850.072.627472
GTAGGGT7050.071.999723
TAGGGTA5650.070.707684
AGAGGGC22700.070.189153
GTAGGGC7250.070.013513
TATAGGG11850.069.8056262
AGGGCAT14300.069.678045
ATAGGGC10850.068.8753053
AGTAAGG10100.068.433541
TAAGGGA12850.068.3966143
TAGAGGG27550.068.068692
ACGGGTA1450.068.068694
AGGGATC13750.067.6797265
AGGGAAT16250.067.6797265
TAGCGGG8000.067.5622252
CCGTACA19550.067.554723
TCCGTAC20150.067.4091642
Produced by FastQC (version 0.11.5)