FastQCFastQC Report
Mon 27 Feb 2023
SRR3128361.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128361.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382465
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176851.2792367257037247No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG34760.25143493687001117No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC33480.2421761129576517No Hit
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG30230.21866738036767658No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGCGTCTAA23480.16984155114234356No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT17130.12390910438962288No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66850.085.236261
GTAGGGC7050.080.7911153
ACGGGTA2100.076.212634
TAGGGCA7800.075.436714
GGTAAGG8900.075.134391
AGTAGGG22050.073.867012
CGTAGGG3900.073.62892
CGGGTAT2000.072.862085
AGGGTAC7250.071.970565
GTAGGGT6150.071.1828163
AGAGGGC17650.070.942143
TAGGGTA4850.070.85144
ATAACGG1800.070.636911
TATAGGG10550.069.607372
AGGGCAT11900.068.734095
CGAAGGG12900.068.604282
TAGGGCG2900.068.173974
AGGGATG19000.068.03765
AAGGGTA9700.067.93974
ATAGGGA11800.067.816323
Produced by FastQC (version 0.11.5)