FastQCFastQC Report
Mon 27 Feb 2023
SRR3128360.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128360.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences892505
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTATGCTCA156771.7565167702141726No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110741.2407773625918062No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG72860.8163539700057701No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC68230.7644775099299164No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATG54640.6122094554092133No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG46420.5201091310412828No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA45090.5052072537408754No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCTATGCTCATC36470.40862516176379965No Hit
ACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG23950.2683458355975597No Hit
CGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT22830.2557968862919536No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCTATGCTCATCGT21470.24055887642086038No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGC20780.23282782729508517No Hit
AGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT15930.1784863950342015No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG15000.16806628534293927No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG14970.1677301527722534No Hit
ATCCGTACCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCG14680.16448087125562322No Hit
AAAGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT12980.14543335891675677No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG12600.141175679688069No Hit
AGAGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT11520.12907490714337735No Hit
ACCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT11500.1288508187629201No Hit
GGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT10560.11831866488142924No Hit
GAATCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT10320.1156296043159422No Hit
GAAGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT10090.1130525879406838No Hit
ATCCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTC9940.11137192508725441No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCTATGCTCATCG9490.10632993652696623No Hit
GGAGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT9130.10229634567873569No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT58050.087.446681
ACGGGTA800.082.247744
ATAGCGG1150.081.81941
CCGTACA57800.081.800533
CGTACAC57550.081.5842064
TCCGTAC60500.081.101912
ATCCGTA60950.080.815951
TAAGGGA5500.079.470553
TACACCC54950.079.286276
GTACACC58900.079.0759355
AATGCGG2250.077.3647841
AGGGATG12650.077.278515
AGGGAAT8150.076.697285
TAGCGGG2850.075.857572
GAATAGG5050.075.460171
TAGGGTA1700.074.645014
ACTAAGG2050.073.43791
GAGGGAT9800.073.3755344
CGAAGGG5400.073.10912
CGAGGGA4200.072.73613
Produced by FastQC (version 0.11.5)