FastQCFastQC Report
Mon 27 Feb 2023
SRR3128359.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128359.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences967298
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139651.4437122789460952No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTATGCTCA91250.943349412487155No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG84310.8716031667593648No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC76210.7878647531577653No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG48820.5047048582753195No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA32040.33123194713521587No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATG29360.303525904116415No Hit
CGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT24000.24811381807881336No Hit
ACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG20570.2126542182450496No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCTATGCTCATC20070.20748518036840768No Hit
AGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT17350.17936561431947548No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG16480.1703714884141185No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG15450.15972327038823608No Hit
AAAGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT15060.1556914208444554No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG14260.14742096024182827No Hit
AGAGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT12450.1287090431283844No Hit
ACCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT12070.12478057434213655No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCTATGCTCATCGT11450.11837096737510053No Hit
GGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT11150.11526954464911537No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGC11060.11433911783131984No Hit
GAATCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT10690.11051402980260477No Hit
GAAGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT10390.10741260707661962No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCTATGCTCATCG10090.10431118435063444No Hit
TCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG9890.10224356919997767No Hit
CGTTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT9820.10151990389724781No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT68250.087.42051
CGTAGGG2150.083.069632
TAGGGTA2700.080.0739444
ACGGGTA900.078.3332064
AGTAAGG6950.077.797811
TAGCGGG4000.077.549872
AGGGTAT4500.077.2887655
TAGGGCA5200.076.82684
AGGGATG12500.075.5758745
GAATAGG5600.075.5629351
GTAGGGT2250.075.1998753
GACCGAT507.094059E-1175.195998
AATGCGG2450.074.8432851
GGGTATC2200.074.7726066
AGGGAAT11000.073.918055
TACGGGG4550.073.340543
TAAGGGA7800.072.910133
TAGAAGG6350.072.561571
ACGTAGG1300.072.333751
CGAGGGA4700.071.999883
Produced by FastQC (version 0.11.5)