FastQCFastQC Report
Mon 27 Feb 2023
SRR3128358.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128358.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836306
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120511.4409797370818815No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG68940.8243394164336978No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC62190.7436273325792233No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTATGCTCA49400.5906928803571898No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG41710.4987408914918702No Hit
CGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT20870.24954980593227838No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA18010.2153517970694937No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG17540.20973184456407104No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG16340.19538302965660895No Hit
AGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT14690.17565340915884856No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATG14630.17493596841347545No Hit
ACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG13530.16178288808163518No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG13220.1580761108972075No Hit
AAAGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT12240.14635791205611343No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCTATGCTCATC10990.13141122986084042No Hit
AGAGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT10540.12603042427054212No Hit
GGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT10090.12064961868024383No Hit
ACCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT9940.11885601681681107No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCTATGCTCATCG9590.11467094580213462No Hit
GAAGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT9390.11227947665089094No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGGG9210.11012715441477162No Hit
GAATCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT9150.10940971366939851No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGG8910.10653995068790609No Hit
CGTTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT8600.10283317350347838No Hit
GGAGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT8370.10008298397954817No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA156.9030514E-493.994868
CGTTTTT58900.087.770021
ACGGGTA900.083.6159744
AGGGTAC3250.082.436125
GTAGGGT1400.080.629693
CGTAGGG2250.079.435172
AGTAAGG4200.078.399361
GACCGAT301.4562665E-678.329058
TAGAGGG10000.078.076422
TAAGGGA7250.077.849363
TAGGGTA2000.077.606074
GTACGGG1700.077.467742
AGGGCAT6450.075.7878655
TAGGGCA4750.075.254374
TATAAGG3200.074.969391
CGAGGGA3650.074.738943
AATGCGG1400.073.9193951
TACGGGG3700.073.728953
GGATGGC9600.073.433487
CGGGTAT900.073.115855
Produced by FastQC (version 0.11.5)