FastQCFastQC Report
Mon 27 Feb 2023
SRR3128357.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128357.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1354990
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175861.2978693569694242No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGACACAAA104010.7676071410121108No Hit
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG56670.41823186886988095No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC53340.39365604174200547No Hit
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG42810.3159432910943992No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA42600.3143934641584071No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGACACAAATCGTATG33120.24442984819076155No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCGACACAAATC22180.1636912449538373No Hit
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT19020.1403700396312888No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC18120.13372792419132246No Hit
ACTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG16260.12000088561539199No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCGACACAAATCGT14680.10834028295411775No Hit
AGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT14010.10339559701547613No Hit
AAAGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTT14000.10332179573280985No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT65700.087.297261
ACGGGTA1050.085.0349354
CCGTACA41000.079.3149953
TCCGTAC43500.078.105582
ATCCGTA43700.077.9505841
CGTACAC41350.077.848124
GGTAAGG9100.076.521341
TAGCGGG4750.075.188782
ATAACGG1200.074.4962841
TACACCC41100.073.976796
CGAAGGG12900.073.5859152
AGGGATG17700.073.54275
CGTAGGG3800.072.962792
GTACACC44000.072.8391345
AGTAAGG7550.072.2891851
AGAGGGC16450.070.5609053
GAGGGAT16450.070.5609054
GTCGATC200.002164343670.489488
AAGAGGG43500.069.7872852
AGTAGGG15700.069.4418642
Produced by FastQC (version 0.11.5)