FastQCFastQC Report
Mon 27 Feb 2023
SRR3128356.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128356.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1488515
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218211.465957682656876No Hit
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG65150.4376845379455363No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGACACAAA60150.4040940131607676No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC59010.39643537350984037No Hit
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG47820.32125977904152797No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA29030.19502658690036714No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC19890.13362310759380994No Hit
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT19870.13348874549467088No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGACACAAATCGTATG17900.12025407872947198No Hit
ACTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG15380.10332445423794855No Hit
AGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT14950.10043566910645846No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT77850.088.259631
AGGGATG20550.078.449485
GTAGGGA9500.074.2147143
AGTAGGG18100.072.711292
CGAAGGG12600.072.369172
ATAGCGG2750.071.826891
ATAGGGA13250.071.6568763
TAGCGGG6800.071.19522
AAGGGTA8950.070.897864
CGTAGGG4250.070.780472
AAGGGAT23700.070.008184
GAGGGAT17450.069.763254
GAATAGG7550.069.142721
CCGTACA26950.069.0651253
AGGGCAT10900.068.992415
GTAAAGG9550.068.451341
ACGGGTA1100.068.36754
ATGCGGG7300.068.250432
AGGGTAT9050.068.0347065
TAGAGGG24650.067.691452