FastQCFastQC Report
Mon 27 Feb 2023
SRR3128354.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128354.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270919
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159491.2549186848257048No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCATGCCTGT80590.6341080745507779No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG62980.4955469231320013No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC58120.4573068779363594No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG53650.42213547834283693No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA32180.25320260378513504No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATG28730.22605689268946327No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT18950.14910470297477652No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCATGCCTGTTC18680.14698025601946307No Hit
ACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG18620.1465081566960601No Hit
CGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT18440.14509185872585115No Hit
AGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT17860.1405282319329556No Hit
AGAGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT14960.11771009796847792No Hit
GGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT14700.11566433423373167No Hit
GGAGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT14460.1137759369401197No Hit
AAAGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT13200.103861851148657No Hit
GAAGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT13060.1027602860607167No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC12920.1016587209727764No Hit
ACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT12880.10134398809050772No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT59350.085.3780441
ACGGGTA1250.078.955414
GTAGGGC5600.077.20983
TAGGGTA6100.077.044694
AGGGCAT9350.076.401985
AGGGTAC6900.076.2854165
ATAGCGG2100.076.174721
TCCGTAC34850.075.923842
CCGTACA33150.075.7063
AGGGTAA6150.075.654145
TAGGGCA6150.075.654144
TAGGGCG2300.075.60434
CGTACAC33050.075.3662644
AGTAAGG6150.074.972541
ATCCGTA35400.074.959571
TAGCGGG4650.074.791342
GTAGGGT6100.074.733353
ATAGGGA10150.073.621333
AAGGGTA7500.072.062474
CGAAGGG11700.071.5001142
Produced by FastQC (version 0.11.5)