FastQCFastQC Report
Mon 27 Feb 2023
SRR3128353.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128353.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1390043
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197371.4198841330807752No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG69530.5002003535142437No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC63810.4590505473571681No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG56490.4063903059113998No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCATGCCTGT46030.3311408352115726No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA21490.15459953397125126No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT21210.15258520779573007No Hit
CGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT19920.1433049193442217No Hit
AGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT18890.1358950766271259No Hit
ACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG16710.12021210854628239No Hit
AGAGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT15870.1141691300197188No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATG15700.11294614627029523No Hit
AAAGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT15340.11035629833033941No Hit
GGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT15240.10963689612479613No Hit
GGAGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT14640.10532048289153646No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71550.086.4901351
GTAGGGT7150.080.2080153
TAGGGTA7150.075.605924
TAGGGCA7150.075.605924
AGGGCAT11700.072.318715
CGTAGGG4050.071.961582
AGGGAAT15500.071.8754655
GGTAAGG7800.070.536121
CGAAGGG13350.070.422712
ATAGGGC8150.070.366553
AGAGGGC18050.070.3154143
TAAGGGA11500.070.306363
TGTAGGG8000.069.923152
ATAAGGG14950.069.8032762
AGGGTAC6150.069.5553055
ATAGCGG2450.069.09661
TAGGGCG2250.068.943834
AGTAGGG21850.068.62832
AGGGCGT2200.068.3740545
AGTAAGG7600.068.0611651
Produced by FastQC (version 0.11.5)