FastQCFastQC Report
Mon 27 Feb 2023
SRR3128352.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128352.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1219241
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171001.4025118905942304No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG58600.480626881805976No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC52160.4278071357508483No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG48150.3949178218252175No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCATGCCTGT25350.20791623641265344No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT18980.15567061803203797No Hit
CGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT18210.14935521361240314No Hit
AGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT15870.13016294563585049No Hit
GGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT14380.11794222799266102No Hit
GGAGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT13350.10949434935340922No Hit
ACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG13320.1092482946357611No Hit
AGAGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT13090.10736187513379225No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC12360.10137454367102157No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66050.086.0204851
TAGGGCG1700.080.27714
AGGGTAC6550.076.056595
ACGGGTA1250.075.294384
TAGGGTA6250.075.294384
TAGCGGG4200.075.070292
TAGGGCA7000.074.6221164
AGGGAAT13800.073.220325
TACGGGT900.073.202873
TGTAGGG7100.072.245492
GTAGGGT6450.072.230083
ATAGGGA10550.071.815143
AGGGCAT9750.071.339385
GGTAAGG5850.070.8099441
AGGGTAA6000.070.495855
AAGAGGG36050.070.360052
CGAAGGG10300.070.360042
GTAGGGC4950.070.3508153
CGTAGGG3350.070.23732
ATAGGGC7550.069.809363
Produced by FastQC (version 0.11.5)