FastQCFastQC Report
Mon 27 Feb 2023
SRR3128351.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128351.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences669316
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAGTTGTGG127541.9055274339773738No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82131.2270736094759427No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG57000.8516156792904995No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC48010.7172994519778401No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATG42740.6385623532083501No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA38440.5743176616127509No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG35110.5245653771910428No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCAGTTGTGGTC23050.34438142820431605No Hit
ACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG18070.2699771109610408No Hit
CGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT16960.2533930161538048No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGC16620.2483132033299667No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGT16260.24293457798707938No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC13430.20065260654160366No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG12960.19363051234394515No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG12600.1882518870010578No Hit
ATCCGTACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCG11100.16584094807236044No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG10620.1586694476151773No Hit
AGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT10550.15762360379850474No Hit
AAAGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTT9490.14178654028889195No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCG9360.13984425891507152No Hit
ATCCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTC8520.12729413311500098No Hit
AGAGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTT8170.12206491403163827No Hit
GGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT7940.11862857006257135No Hit
ACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT7900.11803094502447274No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC7410.11071003830776495No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGG7260.1084689444148952No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTT7010.10473378792677898No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGGG6920.10338913159105714No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTT6920.10338913159105714No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT47000.086.58741
AGTACGG500.084.685481
CGTAGGG1450.081.031522
GTCACGG353.8351573E-880.652831
TAGCGGG1900.079.155012
GTACGGG900.078.3304752
AGGGATG8850.078.064945
AGGGCAT6050.077.683115
CCGTACA47500.077.472963
TCCGTAC50050.077.3757862
ATCCGTA50350.077.275611
CGTACAC47700.077.1481254
GGGCATC3600.077.024966
AGGGTAC2750.076.906285
GAATAGG2650.076.341211
AGGGCGC1850.076.213435
TAAGAGG3150.076.172121
GTACACC48250.075.392075
ATTGCGG255.5299337E-575.275981
GGTACTC507.094059E-1175.197257
Produced by FastQC (version 0.11.5)