FastQCFastQC Report
Mon 27 Feb 2023
SRR3128350.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128350.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722278
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99881.3828470478126151No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAGTTGTGG73551.0183059708311757No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG64500.8930079553855994No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC52850.7317127200330067No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG38330.5306820919368996No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA26800.3710482667338615No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATG22550.3122066572704693No Hit
CGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT18780.26001068840529545No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC15860.21958304143280014No Hit
ACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG13840.19161597058196428No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG12720.17610947585278797No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG12340.17084834371253174No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG12200.1689100318713847No Hit
AGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT11520.15949537435724195No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCAGTTGTGGTC10900.1509114219178765No Hit
AAAGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTT10180.14094296102054887No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCG9930.13748168987564346No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGC9360.12958999166525909No Hit
AGAGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTT9200.12737477813251963No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGT8570.11865237484735794No Hit
ACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT8290.11477575116506386No Hit
GGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT8170.11311434101550927No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTT7940.10992997156219628No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTT7810.10813011056684545No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC7720.10688405295467951No Hit
GAAGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTT7490.1036996835013665No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA700.093.998344
CGTTTTT53400.087.810171
CGTAGGG1650.082.60462
AGGGTAT4500.081.465235
TAGGGCA2900.081.033054
GTACGAT301.4551442E-678.337378
GAATAGG3400.077.464021
TTACGGG1650.076.907732
AAGGGTA4900.076.733344
AGGGATG9550.076.281375
TAGGGTA2650.076.26284
AGGGCGT1050.076.0938955
AGGGTAC3100.074.289015
ATAGCGG950.074.260621
AGGGAAT9100.073.8558355
GCAAGGG9500.073.2197652
GGTACTC1100.072.640117
CAAGGGC4150.072.4806443
TAAGGGA6000.072.065393
AATGCGG1850.071.183161
Produced by FastQC (version 0.11.5)