FastQCFastQC Report
Mon 27 Feb 2023
SRR3128349.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128349.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633288
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87601.3832569068101717No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG53970.8522188956683215No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC43570.6879966144945112No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAGTTGTGG38140.6022536349970313No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG31970.5048256085698766No Hit
CGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT16960.267808643144983No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA14750.2329114083955483No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG14480.22864794532661287No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC13350.21080456285291999No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG13130.20733062998193555No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATG12630.19943532800242547No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG11640.1838026300829954No Hit
AGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT9920.15664279127348063No Hit
ACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG9400.14843167721479011No Hit
AAAGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTT9120.14401030810626445No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCG8610.13595710008716413No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGG8240.13011457662232664No Hit
AGAGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTT8040.1269564558305226No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGGG7100.1121132881090436No Hit
GGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT6850.10816563711928853No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTT6830.10784982504010814No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTT6710.10595495256502571No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC6600.10421798612953348No Hit
ACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT6430.10153358345650004No Hit
GAAGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTT6380.10074405325854903No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTC450.093.9939967
CGTTTTT49600.087.6013641
ATAGCGG900.083.688991
AGTAAGG3200.082.381351
TCCGCTA3100.082.001711
CGTAGGG1400.080.668192
TAGCGGG1550.078.9333952
TAAGGGA5550.078.851353
AGAGGGC7500.078.4274143
GGGTACG600.078.328336
TAGGGCA2750.077.0014654
AAGGGAT11550.077.001464
AGGGAAT7900.076.7419365
TAGCAGG2100.076.216761
AGGGATG10000.076.135145
CGGGTAG255.5592092E-575.19525
GGGCGAT1000.075.19526
CGAAGGG4600.074.676542
AATGCGG1450.074.6707841
AGGGCAT5100.074.6422965
Produced by FastQC (version 0.11.5)