FastQCFastQC Report
Mon 27 Feb 2023
SRR3128348.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128348.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366502
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196551.4383440346227083No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTTTACCT96100.7032554654146134No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG78000.5708004818141502No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC76830.562238474586938No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG68550.5016458080558975No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA34250.2506399551555724No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATG33130.24244384567311283No Hit
ACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG24250.1774604062050403No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCCTTTACCTTC22690.1660443965687573No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT21940.1605559303974674No Hit
AGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT21700.15879962122265465No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT20390.14921310031013493No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC18710.13691893608644554No Hit
AGAGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT18250.13355267683472105No Hit
GGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT17970.13150364946410617No Hit
AAAGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT17850.13062549487669978No Hit
ACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT16120.11796543290825771No Hit
GGAGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT15820.11577004643974177No Hit
GAAGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT15080.11035475981740238No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGT14780.10815937334888644No Hit
GGAACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT14250.10428085725450822No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGTA4850.087.2118454
CGTTTTT65200.086.461851
ACGGGTA1600.085.182954
TAGGGCA6100.083.9791264
TAGGGCG2300.083.778144
GTAGGGT5300.081.580563
CGTAGGG2600.077.726622
AGGGTAA6750.076.588515
ATAGCGG2550.075.658721
AGGGCAT10150.075.473815
AGGGAAT12800.075.269425
CCGTACA39700.075.053933
ATAGGGC8100.074.847863
ATCCGTA41750.074.838811
TCCGTAC41600.074.789282
AGGGTAC7300.074.6809545
AAGGGTA10150.074.5477454
CGTACAC39950.074.466614
GTAGGGC5250.074.30083
TAGCGGG6300.073.85322
Produced by FastQC (version 0.11.5)