FastQCFastQC Report
Mon 27 Feb 2023
SRR3128347.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128347.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495072
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237441.5881509385501167No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG89030.5954897155454721No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC86050.5755575651206096No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG75040.5019156268059332No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTTTACCT54550.3648653710322981No Hit
AGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT23930.16005918109629502No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT23240.15544401874959868No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23240.15544401874959868No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA23020.15397251771152157No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC22550.15082885640290233No Hit
ACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG22350.14949112818646862No Hit
AGAGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT20400.1364482780762398No Hit
AAAGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT19830.1326357526594037No Hit
GGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT18720.12521136105819652No Hit
ACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT18640.12467626977162305No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATG16850.1127036022345412No Hit
GGAGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT16030.10721891654716295No Hit
GAAGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT15600.10434280088183046No Hit
GGAACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT15570.10414214164936539No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT79950.087.398531
TAGGGTA5900.086.831014
TAGGGCA6750.081.4671254
AGGGTAC6550.081.0844355
ATAGCGG3450.077.6889951
AGGGCAT13000.075.923515
GAGGGTA7100.075.465224
AGTAAGG9250.074.72731
TAAGGGA11900.074.647483
ACGGGTA1450.074.5521554
ATAGGGA11750.074.400433
GTAGGGT7150.074.2801363
AGGGATG19550.073.8060465
GGTAAGG8700.073.5060961
ATAGAGG7250.073.28991
TAGCGGG8100.073.111532
CGTAGGG3800.072.97412
AGGGTAA8900.072.876825
GTAGGGA10300.072.097493
TAGGGAC8300.071.916084
Produced by FastQC (version 0.11.5)