FastQCFastQC Report
Mon 27 Feb 2023
SRR3128346.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128346.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1313891
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204541.55675014137398No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG74030.5634409551477254No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC68300.51983003156274No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG62180.4732508252206614No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTTTACCT28110.21394468795356691No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT21530.163864430154404No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT21450.16325555164012845No Hit
AGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT20900.15906951185448412No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC19090.1452936354690001No Hit
AGAGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT17460.132887735740636No Hit
GGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT16400.12482009542648514No Hit
ACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG16130.12276513044080521No Hit
AAAGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT16040.12208014211224523No Hit
GGAGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT15420.1173613336266098No Hit
ACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT14450.10997868164101891No Hit
GAAGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT13960.10624930074108127No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT68650.088.243751
TAGGGTA5650.085.789764
AGGGTAC7300.081.7676855
TAGGGCA6200.081.215464
AGGGTAA6150.081.008695
ACGGGTA1750.080.673314
GTAGGGT6200.078.938393
CGTAGGG2950.076.571272
GTAGGGC5500.075.295093
GTAGGGA9150.075.089363
TAACGGG2700.074.9464952
AGAGGGT13200.074.154253
GAGGGTA6000.073.726434
TAGGGAC7250.073.34784
ATAGAGG6250.073.052941
ATAGGGA11050.072.399123
AGGGATG14850.072.162125
AAGGGTA8950.072.03514
AGGGCAT11200.071.7594155
GGGTACC9000.071.544946
Produced by FastQC (version 0.11.5)