FastQCFastQC Report
Mon 27 Feb 2023
SRR3128345.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128345.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2057836
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256121.2446084138872098No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAGGGAAGC47060.22868683413061097No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34540.16784622292544207No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA34090.16565945974314766No Hit
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG33590.16322972287393164Illumina PCR Primer Index 8 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG33360.16211204391409229Illumina PCR Primer Index 8 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC31430.15273325959891848Illumina PCR Primer Index 8 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC26510.12882464880583291No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT80800.084.2465061
TAGGGTA7600.072.34754
GAGGGAT24900.072.285424
AGGGTAC12250.072.122885
CCGTACA20200.071.190383
AGGGATG29300.070.893425
AGTAGGG34800.070.762522
TAGGGCA11750.069.992494
GAATAGG9850.069.728661
ACGGGAT6350.069.5673454
CGTAGGG6300.069.373512
AGGGCAT19950.069.255735
ATAGCGG4300.068.9234161
ATAGGGA17050.068.907563
AGACGGG13800.068.449172
TCCGTAC21800.068.336712
ATAAGGG32200.068.303232
GGTAAGG12600.067.950951
TACGGGT1400.067.135653
ACGGGTA2800.067.135654