Basic Statistics
Measure | Value |
---|---|
Filename | SRR3128345.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2057836 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 25612 | 1.2446084138872098 | No Hit |
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAGGGAAGC | 4706 | 0.22868683413061097 | No Hit |
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT | 3454 | 0.16784622292544207 | No Hit |
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA | 3409 | 0.16565945974314766 | No Hit |
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG | 3359 | 0.16322972287393164 | Illumina PCR Primer Index 8 (95% over 22bp) |
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG | 3336 | 0.16211204391409229 | Illumina PCR Primer Index 8 (95% over 22bp) |
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC | 3143 | 0.15273325959891848 | Illumina PCR Primer Index 8 (95% over 23bp) |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 2651 | 0.12882464880583291 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 8080 | 0.0 | 84.246506 | 1 |
TAGGGTA | 760 | 0.0 | 72.3475 | 4 |
GAGGGAT | 2490 | 0.0 | 72.28542 | 4 |
AGGGTAC | 1225 | 0.0 | 72.12288 | 5 |
CCGTACA | 2020 | 0.0 | 71.19038 | 3 |
AGGGATG | 2930 | 0.0 | 70.89342 | 5 |
AGTAGGG | 3480 | 0.0 | 70.76252 | 2 |
TAGGGCA | 1175 | 0.0 | 69.99249 | 4 |
GAATAGG | 985 | 0.0 | 69.72866 | 1 |
ACGGGAT | 635 | 0.0 | 69.567345 | 4 |
CGTAGGG | 630 | 0.0 | 69.37351 | 2 |
AGGGCAT | 1995 | 0.0 | 69.25573 | 5 |
ATAGCGG | 430 | 0.0 | 68.923416 | 1 |
ATAGGGA | 1705 | 0.0 | 68.90756 | 3 |
AGACGGG | 1380 | 0.0 | 68.44917 | 2 |
TCCGTAC | 2180 | 0.0 | 68.33671 | 2 |
ATAAGGG | 3220 | 0.0 | 68.30323 | 2 |
GGTAAGG | 1260 | 0.0 | 67.95095 | 1 |
TACGGGT | 140 | 0.0 | 67.13565 | 3 |
ACGGGTA | 280 | 0.0 | 67.13565 | 4 |