FastQCFastQC Report
Mon 27 Feb 2023
SRR3128344.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128344.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2291012
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT306441.337574835924037No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38950.17001220421368374No Hit
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG37990.1658219162536032Illumina PCR Primer Index 8 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG34780.15181064088708396Illumina PCR Primer Index 8 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC34040.14858062725118856Illumina PCR Primer Index 8 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30270.132125017241289No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAGGGAAGC27730.12103821368024262No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA24310.10611031282245575No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT96650.085.45211
TAGGGTA11450.076.762964
TAGGGCA12850.072.788964
GTAGGGT12000.071.6779253
GAGGGAT26350.070.9934164
AGGGTAC13850.070.927095
ATAGGGA20100.070.853643
AGGGATG33550.070.327765
AGGGCAT20950.069.9980855
CGTAGGG7600.069.884432
TAGGGCG3900.068.6951144
CCGTACA16300.068.340223
AAGGGTA16950.067.6605764
AGATAGG15300.067.628741
TAAGGGA19200.067.320463
AGTAGGG40400.066.896292
TAGAGGG43850.066.77812
AGGGAAT25350.066.748285
TAGAAGG18150.066.597291
GGTAAGG14100.066.379511