Basic Statistics
Measure | Value |
---|---|
Filename | SRR3128344.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2291012 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 30644 | 1.337574835924037 | No Hit |
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT | 3895 | 0.17001220421368374 | No Hit |
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG | 3799 | 0.1658219162536032 | Illumina PCR Primer Index 8 (95% over 22bp) |
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG | 3478 | 0.15181064088708396 | Illumina PCR Primer Index 8 (95% over 22bp) |
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC | 3404 | 0.14858062725118856 | Illumina PCR Primer Index 8 (95% over 23bp) |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 3027 | 0.132125017241289 | No Hit |
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAGGGAAGC | 2773 | 0.12103821368024262 | No Hit |
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA | 2431 | 0.10611031282245575 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 9665 | 0.0 | 85.4521 | 1 |
TAGGGTA | 1145 | 0.0 | 76.76296 | 4 |
TAGGGCA | 1285 | 0.0 | 72.78896 | 4 |
GTAGGGT | 1200 | 0.0 | 71.677925 | 3 |
GAGGGAT | 2635 | 0.0 | 70.993416 | 4 |
AGGGTAC | 1385 | 0.0 | 70.92709 | 5 |
ATAGGGA | 2010 | 0.0 | 70.85364 | 3 |
AGGGATG | 3355 | 0.0 | 70.32776 | 5 |
AGGGCAT | 2095 | 0.0 | 69.998085 | 5 |
CGTAGGG | 760 | 0.0 | 69.88443 | 2 |
TAGGGCG | 390 | 0.0 | 68.695114 | 4 |
CCGTACA | 1630 | 0.0 | 68.34022 | 3 |
AAGGGTA | 1695 | 0.0 | 67.660576 | 4 |
AGATAGG | 1530 | 0.0 | 67.62874 | 1 |
TAAGGGA | 1920 | 0.0 | 67.32046 | 3 |
AGTAGGG | 4040 | 0.0 | 66.89629 | 2 |
TAGAGGG | 4385 | 0.0 | 66.7781 | 2 |
AGGGAAT | 2535 | 0.0 | 66.74828 | 5 |
TAGAAGG | 1815 | 0.0 | 66.59729 | 1 |
GGTAAGG | 1410 | 0.0 | 66.37951 | 1 |