FastQCFastQC Report
Mon 27 Feb 2023
SRR3128343.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128343.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1981076
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268741.3565355392725973No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT35290.17813551827390772No Hit
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG30290.1528967086573155Illumina PCR Primer Index 8 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG30240.1526443205611496Illumina PCR Primer Index 8 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC29120.14699082720703294Illumina PCR Primer Index 8 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23460.1184204947210506No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT84300.085.108271
TAGGGTA8750.076.907154
AGGGTAC12400.073.540255
CGTAGGG7000.073.278772
TAGACGG2650.072.837041
GAATAGG10450.072.080541
ATAGGGA15750.071.1107253
TAGGGCA12300.070.7791444
AGACGGG13500.070.763942
AAGGGTA14250.070.670414
ACGGGAT6450.070.040664
GTAGGGT9000.069.542113
AGGGCAT19500.069.422935
GAGGGAT23750.069.3494644
AGTAGGG35100.069.181872
GGTAAGG10450.068.927021
ATAGCGG3850.068.476521
TAGAGGG36250.068.285332
AGGGATG27800.067.802345
CGAAGGG21100.067.801742
Produced by FastQC (version 0.11.5)