FastQCFastQC Report
Mon 27 Feb 2023
SRR3128342.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128342.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2173643
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241061.109013761689477No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGCATGATT71640.32958494104137614No Hit
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG53950.24820083150728983Illumina PCR Primer Index 5 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC52610.24203606572008374Illumina PCR Primer Index 5 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG50130.23062664844226952Illumina PCR Primer Index 5 (95% over 22bp)
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA46070.21194832822133167No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24600.11317405848154459No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23950.11018368701760133No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT90250.085.822131
CCGTACA30400.075.5930943
TCCGTAC31500.075.489552
ATCCGTA31850.075.201241
AGGGTAT12850.073.508875
CGTACAC31000.073.3720254
AAGGGTA13600.072.5654
CGTAGGG4800.072.449832
AGGGATG28750.072.085445
TAAGGGA16200.071.942173
TAGGGTA7300.070.813594
GGTAAGG12600.070.584261
ATAGCGG3750.070.270561
TAGGGCA12550.070.023644
AGGGCAT18600.069.98645
AGTAAGG14800.068.994541
GTAGGGC9400.068.491963
AGTAGGG33750.068.0897752
GTACACC33000.068.0707865
AGAGGGC30250.068.04493
Produced by FastQC (version 0.11.5)