FastQCFastQC Report
Mon 27 Feb 2023
SRR3128341.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128341.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2405337
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294561.224610106608762No Hit
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG60340.2508588193670991Illumina PCR Primer Index 5 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC58570.24350018313442148Illumina PCR Primer Index 5 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG56000.23281560962143769Illumina PCR Primer Index 5 (95% over 22bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGCATGATT39030.1622641650629413No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA32350.13449258877238407No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28620.11898540620295618No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26350.1095480591700872No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT105800.084.64031
CGTAGGG6000.076.769862
AGGGTAT14000.072.8500065
ACGGGTA1950.072.309194
AGGGCAT23350.072.059955
TAGGGCA16650.071.983484
AGTAAGG15650.071.525111
TAGGGTA7250.071.311834
GGTAAGG13900.070.0408551
TAGAGGG42800.069.294942
AGTAGGG40400.069.2231752
ATAGGGC17950.068.866373
AGGGATG32150.068.562995
GAATAGG13700.068.316941
AGAGGGC34500.067.573823
AAGGGTA16400.067.348964
AGGGAAT21000.066.9190445
GTAGGGC10700.066.769133
TGAAGGG38400.066.58612
CGAAGGG20900.066.567362