Basic Statistics
Measure | Value |
---|---|
Filename | SRR3128341.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2405337 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 29456 | 1.224610106608762 | No Hit |
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG | 6034 | 0.2508588193670991 | Illumina PCR Primer Index 5 (95% over 22bp) |
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC | 5857 | 0.24350018313442148 | Illumina PCR Primer Index 5 (95% over 23bp) |
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG | 5600 | 0.23281560962143769 | Illumina PCR Primer Index 5 (95% over 22bp) |
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGCATGATT | 3903 | 0.1622641650629413 | No Hit |
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA | 3235 | 0.13449258877238407 | No Hit |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 2862 | 0.11898540620295618 | No Hit |
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT | 2635 | 0.1095480591700872 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 10580 | 0.0 | 84.6403 | 1 |
CGTAGGG | 600 | 0.0 | 76.76986 | 2 |
AGGGTAT | 1400 | 0.0 | 72.850006 | 5 |
ACGGGTA | 195 | 0.0 | 72.30919 | 4 |
AGGGCAT | 2335 | 0.0 | 72.05995 | 5 |
TAGGGCA | 1665 | 0.0 | 71.98348 | 4 |
AGTAAGG | 1565 | 0.0 | 71.52511 | 1 |
TAGGGTA | 725 | 0.0 | 71.31183 | 4 |
GGTAAGG | 1390 | 0.0 | 70.040855 | 1 |
TAGAGGG | 4280 | 0.0 | 69.29494 | 2 |
AGTAGGG | 4040 | 0.0 | 69.223175 | 2 |
ATAGGGC | 1795 | 0.0 | 68.86637 | 3 |
AGGGATG | 3215 | 0.0 | 68.56299 | 5 |
GAATAGG | 1370 | 0.0 | 68.31694 | 1 |
AGAGGGC | 3450 | 0.0 | 67.57382 | 3 |
AAGGGTA | 1640 | 0.0 | 67.34896 | 4 |
AGGGAAT | 2100 | 0.0 | 66.919044 | 5 |
GTAGGGC | 1070 | 0.0 | 66.76913 | 3 |
TGAAGGG | 3840 | 0.0 | 66.5861 | 2 |
CGAAGGG | 2090 | 0.0 | 66.56736 | 2 |