Basic Statistics
Measure | Value |
---|---|
Filename | SRR3128340.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2094812 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 25193 | 1.2026377546051865 | No Hit |
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG | 4751 | 0.226798395273657 | Illumina PCR Primer Index 5 (95% over 22bp) |
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC | 4638 | 0.22140411645531913 | Illumina PCR Primer Index 5 (95% over 23bp) |
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG | 4472 | 0.21347977766023873 | Illumina PCR Primer Index 5 (95% over 22bp) |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 2371 | 0.11318438122370886 | No Hit |
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT | 2276 | 0.1086493680578496 | No Hit |
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGCATGATT | 2138 | 0.10206166472218033 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 9455 | 0.0 | 84.9951 | 1 |
CGTAGGG | 575 | 0.0 | 77.750145 | 2 |
GGTAAGG | 1260 | 0.0 | 72.48584 | 1 |
AGTAAGG | 1290 | 0.0 | 72.2599 | 1 |
AGGGCAT | 1920 | 0.0 | 72.218346 | 5 |
AGGGATG | 2955 | 0.0 | 71.89649 | 5 |
TAGGGCA | 1275 | 0.0 | 70.496666 | 4 |
ATAGCGG | 335 | 0.0 | 70.266304 | 1 |
CGAAGGG | 1945 | 0.0 | 70.165535 | 2 |
AGGGTAA | 925 | 0.0 | 69.61545 | 5 |
TAAGGGA | 1580 | 0.0 | 69.397575 | 3 |
AGTAGGG | 3310 | 0.0 | 69.38048 | 2 |
AAGGGTA | 1360 | 0.0 | 68.5128 | 4 |
TAGAGGG | 3580 | 0.0 | 68.22284 | 2 |
AAGGGAT | 3395 | 0.0 | 67.92063 | 4 |
AGGGTAT | 1060 | 0.0 | 67.84413 | 5 |
ACGGGTA | 160 | 0.0 | 67.64774 | 4 |
AGAGGGC | 3195 | 0.0 | 67.45902 | 3 |
GAGGGTA | 1120 | 0.0 | 67.22757 | 4 |
TATAGGG | 1425 | 0.0 | 67.03886 | 2 |