FastQCFastQC Report
Mon 27 Feb 2023
SRR3128340.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128340.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2094812
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251931.2026377546051865No Hit
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG47510.226798395273657Illumina PCR Primer Index 5 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC46380.22140411645531913Illumina PCR Primer Index 5 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG44720.21347977766023873Illumina PCR Primer Index 5 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23710.11318438122370886No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22760.1086493680578496No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGCATGATT21380.10206166472218033No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT94550.084.99511
CGTAGGG5750.077.7501452
GGTAAGG12600.072.485841
AGTAAGG12900.072.25991
AGGGCAT19200.072.2183465
AGGGATG29550.071.896495
TAGGGCA12750.070.4966664
ATAGCGG3350.070.2663041
CGAAGGG19450.070.1655352
AGGGTAA9250.069.615455
TAAGGGA15800.069.3975753
AGTAGGG33100.069.380482
AAGGGTA13600.068.51284
TAGAGGG35800.068.222842
AAGGGAT33950.067.920634
AGGGTAT10600.067.844135
ACGGGTA1600.067.647744
AGAGGGC31950.067.459023
GAGGGTA11200.067.227574
TATAGGG14250.067.038862