FastQCFastQC Report
Mon 27 Feb 2023
SRR3128339.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128339.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2075279
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296091.4267479216047576No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTAGGAAT153340.7388886024481528No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC115950.5587200564357853No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG93470.45039727188488876No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG85690.412908336662203No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA61520.29644206875316526No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATG42230.20349071136941105No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34610.16677275681968545No Hit
CGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT32090.1546298112205636No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCCTAGGAATTC29860.14388426809118196No Hit
AGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT29310.14123402202788155No Hit
ACTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG26170.12610352632103924No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC25860.124609751267179No Hit
AGAGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTT25720.12393514317833891No Hit
GGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT25230.12157401486739855No Hit
AAAGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTT24010.11569528723607767No Hit
ACCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT23470.11309322746483724No Hit
GGAGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTT22040.1062025877002562No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCCTAGGAATTCGT20910.10075753669747536No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT100500.086.0012741
TAGGGCA8350.079.9191064
CCGTACA56800.077.3592153
CGTACAC56850.076.960524
TCCGTAC59300.076.871582
ATCCGTA60450.075.731811
ATAGGGA15750.074.893123
GAATAGG11500.074.462111
CGTAGGG6300.073.84882
GTAGGGC7750.073.372353
GGTAAGG10750.072.654321
AGGGCAT15350.072.558575
TAGGGTA8600.072.131384
AAGGGTA12400.072.007994
GTAGGGT8500.071.321353
GTACACC60900.071.147955
ATAGAGG11350.070.886251
AGGGTAC9250.070.619045
TAAGGGA13700.070.3205263
TACACCC59350.069.126146
Produced by FastQC (version 0.11.5)