FastQCFastQC Report
Mon 27 Feb 2023
SRR3128338.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128338.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2280113
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT361551.585667026151774No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC131040.5747083587523951No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG104750.459407055702941No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTAGGAAT93070.40818152433673244No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG91450.4010766133082001No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA40710.17854378269848906No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38820.170254719831868No Hit
CGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT34350.15065042829017686No Hit
AGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT32820.1439402345410074No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30140.13218643111108966No Hit
AGAGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTT27840.12209921174959311No Hit
AAAGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTT26840.11771346420111635No Hit
ACTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG26690.11705560206884483No Hit
GGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT26330.11547673295139319No Hit
ACCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT25850.11337157412812436No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATG22980.10078447866399604No Hit
GGAGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTT22840.1001704740072093No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT124200.087.057021
TAGGGTA8650.076.069844
TAGGGCA8100.076.0128254
CGTAGGG6350.075.498192
GGTAAGG12050.074.547441
GTAGGGC8250.074.06273
TCCGCTA7650.072.5449141
AGTAAGG12100.072.295961
GAATAGG12700.072.213321
GTAGGGT8200.071.648363
GAGGGAT26700.071.116554
AGTAGGG31350.070.764352
ATAGAGG11750.070.446791
ATAGGGA18200.070.2437443
GTAGGGA14250.069.924783
TAGCGGG10800.069.63162
AGGGACT32200.069.623135
CCGTACA38200.069.148553
AGGGATG30400.069.107475
CGTACAC38400.068.66454
Produced by FastQC (version 0.11.5)