FastQCFastQC Report
Mon 27 Feb 2023
SRR3128336.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128336.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1498689
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193251.2894603216544593No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAACCCATG50620.33776187054151996No Hit
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG44140.29452408071321RNA PCR Primer, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC42670.28471550802067674RNA PCR Primer, Index 16 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG42140.2811790838526205RNA PCR Primer, Index 14 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29940.19977460300302463No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA28110.18756393087558526No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC17670.11790304726330814No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC16380.1092955242882279No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATG15690.10469150037132455No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT61050.085.571181
ACGGGTA1600.076.369534
TAGGGTA5700.075.854214
AGGGTAC8050.073.5599445
GAGGGAT14650.073.141524
CGTAGGG4150.072.476732
GTACGGG3250.072.302512
AGTAGGG24600.072.214332
CCGTACA21550.071.9673
ATAGGGA11700.071.9008263
GATTAGG4750.071.339341
AGTACGG1650.071.309321
GTAGGGC6200.071.252963
TCCGTAC23150.071.053222
GTAGGGA11200.070.9145663
AGGGCAT11600.070.900095
AGGGTAT7900.070.79245
GAATAGG7250.070.758521
TAGGGCA7050.070.66164
ATCCGTA23750.069.952191