Basic Statistics
Measure | Value |
---|---|
Filename | SRR3128336.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1498689 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 19325 | 1.2894603216544593 | No Hit |
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAACCCATG | 5062 | 0.33776187054151996 | No Hit |
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG | 4414 | 0.29452408071321 | RNA PCR Primer, Index 16 (95% over 22bp) |
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC | 4267 | 0.28471550802067674 | RNA PCR Primer, Index 16 (95% over 23bp) |
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG | 4214 | 0.2811790838526205 | RNA PCR Primer, Index 14 (95% over 22bp) |
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT | 2994 | 0.19977460300302463 | No Hit |
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA | 2811 | 0.18756393087558526 | No Hit |
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC | 1767 | 0.11790304726330814 | No Hit |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 1638 | 0.1092955242882279 | No Hit |
ATCCGTACACCCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATG | 1569 | 0.10469150037132455 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 6105 | 0.0 | 85.57118 | 1 |
ACGGGTA | 160 | 0.0 | 76.36953 | 4 |
TAGGGTA | 570 | 0.0 | 75.85421 | 4 |
AGGGTAC | 805 | 0.0 | 73.559944 | 5 |
GAGGGAT | 1465 | 0.0 | 73.14152 | 4 |
CGTAGGG | 415 | 0.0 | 72.47673 | 2 |
GTACGGG | 325 | 0.0 | 72.30251 | 2 |
AGTAGGG | 2460 | 0.0 | 72.21433 | 2 |
CCGTACA | 2155 | 0.0 | 71.967 | 3 |
ATAGGGA | 1170 | 0.0 | 71.900826 | 3 |
GATTAGG | 475 | 0.0 | 71.33934 | 1 |
AGTACGG | 165 | 0.0 | 71.30932 | 1 |
GTAGGGC | 620 | 0.0 | 71.25296 | 3 |
TCCGTAC | 2315 | 0.0 | 71.05322 | 2 |
GTAGGGA | 1120 | 0.0 | 70.914566 | 3 |
AGGGCAT | 1160 | 0.0 | 70.90009 | 5 |
AGGGTAT | 790 | 0.0 | 70.7924 | 5 |
GAATAGG | 725 | 0.0 | 70.75852 | 1 |
TAGGGCA | 705 | 0.0 | 70.6616 | 4 |
ATCCGTA | 2375 | 0.0 | 69.95219 | 1 |