SRR3128335.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3128335.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1653363
Sequences flagged as poor quality	0
Sequence length	100
%GC	46

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23372	1.413603667192262	No Hit
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG	4926	0.2979382023185471	RNA PCR Primer, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC	4732	0.2862045418943087	RNA PCR Primer, Index 16 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG	4674	0.2826965403241756	RNA PCR Primer, Index 14 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	3499	0.21162926713613403	No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAACCCATG	2920	0.17660973422049484	No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA	1923	0.11630839688562039	No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC	1896	0.11467536167193773	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	7080	85.317055	1
TAGGGCA	780	76.525444	4
GTACGGG	315	76.09734	2
TAGGGTA	585	75.52117	4
GTAGGGC	725	75.20208	3
ACGGGTA	190	74.210335	4
GAATAGG	975	73.34037	1
CGTAGGG	405	71.95261	2
AGGGTAC	850	71.882164	5
AGTAGGG	2470	71.54854	2
ATAGCGG	290	71.377075	1
GATTAGG	510	71.02719	1
TAGGGCG	225	71.02204	4
CCGTACA	1610	70.64792	3
GTAGGGT	800	69.91444	3
AGGGTAT	895	69.8434	5
AAGGGTA	1160	69.689476	4
GTAGGGA	1190	69.51453	3
TGTAGGG	1135	68.74199	2
AATGCGG	370	68.65879	1