FastQCFastQC Report
Mon 27 Feb 2023
SRR3128334.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128334.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1442031
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209141.4503155618707226No Hit
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG41320.28654030322510404RNA PCR Primer, Index 16 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG37790.2620609404374802RNA PCR Primer, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC37710.2615061673431431RNA PCR Primer, Index 16 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31030.21518261396599658No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAACCCATG15430.10700186057026513No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC15330.10630839420234378No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62950.087.641071
GTAGGGC6650.077.838773
TAGGGTA7100.077.544764
ACGGGTA1550.075.898514
GAATAGG9800.075.4135361
AGGGTAC7550.074.0794145
AATGCGG2800.073.972521
AGTAGGG23900.073.4405442
AGGGATG17200.072.959255
GTAGGGT8250.071.868993
ATAGAGG7300.071.577391
ATAAGGG19300.071.195162
AGGGCAT11700.070.70085
AGGGTAA8450.070.6395
TAGGGCA7400.070.585624
TGTAGGG9750.069.982322
GATTAGG4400.069.540281
CGTAGGG3600.069.278472
GCATAGG3550.068.952621
ATAGGGC8450.068.4972843