Basic Statistics
Measure | Value |
---|---|
Filename | SRR3128334.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1442031 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 20914 | 1.4503155618707226 | No Hit |
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG | 4132 | 0.28654030322510404 | RNA PCR Primer, Index 16 (95% over 22bp) |
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG | 3779 | 0.2620609404374802 | RNA PCR Primer, Index 14 (95% over 22bp) |
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC | 3771 | 0.2615061673431431 | RNA PCR Primer, Index 16 (95% over 23bp) |
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT | 3103 | 0.21518261396599658 | No Hit |
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAACCCATG | 1543 | 0.10700186057026513 | No Hit |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 1533 | 0.10630839420234378 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 6295 | 0.0 | 87.64107 | 1 |
GTAGGGC | 665 | 0.0 | 77.83877 | 3 |
TAGGGTA | 710 | 0.0 | 77.54476 | 4 |
ACGGGTA | 155 | 0.0 | 75.89851 | 4 |
GAATAGG | 980 | 0.0 | 75.413536 | 1 |
AGGGTAC | 755 | 0.0 | 74.079414 | 5 |
AATGCGG | 280 | 0.0 | 73.97252 | 1 |
AGTAGGG | 2390 | 0.0 | 73.440544 | 2 |
AGGGATG | 1720 | 0.0 | 72.95925 | 5 |
GTAGGGT | 825 | 0.0 | 71.86899 | 3 |
ATAGAGG | 730 | 0.0 | 71.57739 | 1 |
ATAAGGG | 1930 | 0.0 | 71.19516 | 2 |
AGGGCAT | 1170 | 0.0 | 70.7008 | 5 |
AGGGTAA | 845 | 0.0 | 70.639 | 5 |
TAGGGCA | 740 | 0.0 | 70.58562 | 4 |
TGTAGGG | 975 | 0.0 | 69.98232 | 2 |
GATTAGG | 440 | 0.0 | 69.54028 | 1 |
CGTAGGG | 360 | 0.0 | 69.27847 | 2 |
GCATAGG | 355 | 0.0 | 68.95262 | 1 |
ATAGGGC | 845 | 0.0 | 68.497284 | 3 |