FastQCFastQC Report
Mon 27 Feb 2023
SRR3128333.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128333.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1164630
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153461.3176717068940351No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTCCAGGT74320.6381425860573745No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG47320.4063092999493401No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC46630.4003846715265793No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG39850.34216875745945063No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30460.2615422924018787No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA24040.20641748881619054No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATG24000.20607403209603048No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCCTCCAGGTTC17560.1507775001502623No Hit
CGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT16270.13970102092510067No Hit
AGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT14350.12321509835741824No Hit
ACTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG13890.11926534607557765No Hit
GGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT12190.10466843546877548No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT57150.086.3393861
TAGGGCA5650.083.178214
GTAGGGC4600.077.645053
GTAGGGT5300.076.257153
CGTAGGG2500.075.193092
TAGGGTC3500.075.193094
TAGCGGG4650.074.788832
CCGTACA29450.074.682453
TCCGTAC31150.074.076672
AGGGTAA4900.073.8503655
CGTACAC29750.073.613414
ATAGGGC7150.072.9583363
ATCCGTA31850.072.8093641
AGTAGGG16950.072.0877762
ATAGGGA8750.071.9705353
AGATAGG5450.071.6359941
AGGGTAC6450.070.675695
TGCGTAA200.002163774670.4935394
AGGGATG12500.069.177655
AAGGGAC10200.069.11134
Produced by FastQC (version 0.11.5)