FastQCFastQC Report
Mon 27 Feb 2023
SRR3128331.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128331.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101318
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161971.4706923885744172No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG43720.3969788925632742No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC40120.3642907861307997No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG34560.3138058217517556No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29950.27194688545905904No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTCCAGGT21660.19667344036872186No Hit
CGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT14740.1338396357818541No Hit
AGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT13090.11885758700030329No Hit
GGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT11170.10142393023631685No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT56950.086.028681
GTAGGGC4150.078.2256243
TAGGGCA5450.076.8318944
TAGGGTA3950.076.230884
ATAGGGC6300.074.680553
ATAGGGA9850.074.513753
AGTAGGG15600.073.29082
AGGGAAT9500.073.2171255
GTACGGG2400.072.536782
TAGGGTC3900.072.382684
GTAGGGT5200.071.47793
GAATAGG5100.071.056991
AGGGTAA5200.070.4962165
TATAGGG7650.069.52
ATGAGGG17400.069.49472
TAGCGGG4200.069.456072
GGTAAGG6250.069.2777941
AATGCGG1700.069.211361
TAGAGGG17800.068.990342
AGGGCAT8900.068.648015