FastQCFastQC Report
Mon 27 Feb 2023
SRR3128329.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128329.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2241521
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302901.3513145761293337No Hit
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC58650.2616526902937782Illumina PCR Primer Index 3 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG58150.25942206207302987TruSeq Adapter, Index 14 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT56980.25420239203647876No Hit
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG54000.24090784784081878TruSeq Adapter, Index 14 (95% over 24bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCATTGCGTC31480.14044035277831435No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA25970.11585882978566786No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT92550.084.4380341
TAGGGTA6650.079.8649444
CGTAGGG6250.075.952272
ATAGGGA18050.073.169243
TAGGGCA10050.072.955494
AGTAGGG36950.072.6308442
GGTAAGG12000.072.097471
TAGGGCG4300.071.046764
TATAGGG17100.070.637682
ATAGGGC13500.070.326173
AGGGTAA11500.070.2943345
AGGGAAT17250.070.021875
GAATAGG14600.069.886041
AGGGATG31200.069.444215
TAGACGG2800.068.850851
GTAGGGA18300.068.830853
GAGGGAT26650.068.780734
AGACGGG13400.068.746512
AGGGACT42350.068.14045
GTAGGGC8700.066.988743
Produced by FastQC (version 0.11.5)