FastQCFastQC Report
Mon 27 Feb 2023
SRR3128328.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128328.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1960006
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270621.3807100590508397No Hit
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG51080.260611447107815TruSeq Adapter, Index 14 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT51080.260611447107815No Hit
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC51050.2604583863518785Illumina PCR Primer Index 3 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG48750.2487237283967498TruSeq Adapter, Index 14 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT82950.084.9149251
TAGGGTA6200.080.474994
TAGGGCA10350.075.4943544
GAATAGG11100.073.382411
ACGGGTA1800.073.220434
ATAGGGA16550.073.0940253
AGGGATG27800.073.040675
GTAGGGT7250.072.715463
TAGGGCG3450.072.310864
AGTAAGG12050.071.895151
AGTAGGG30150.071.3469852
AGGGTAG6900.070.845135
GAGGGAT23600.070.804864
ATAGCGG2950.070.226241
AGGGTAA10200.070.0437245
GTAGGGA14450.069.709613
AAGGGTA13450.069.643014
TATAGGG16000.069.1344762
AGGGAAT16000.068.741925
AGAGGGC27050.068.734793
Produced by FastQC (version 0.11.5)