FastQCFastQC Report
Mon 27 Feb 2023
SRR3128327.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128327.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1991058
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270481.3584737360739867No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC34570.17362628311179282No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG33190.16669529466243574No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTACTCGG30830.15484229992295553No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA29830.14981984452487068No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG29220.14675614673203893No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27610.13866999354112236No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC21280.10687785087124532No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT83200.084.7634661
CCGTACA14950.072.6159443
GGTAAGG13400.070.921361
TCCGTAC15950.070.7150342
AGGGATG27750.070.1135
TAGGGCA10400.069.590284
GTAGGGC8050.068.8885963
AGTAGGG33800.068.547462
ATAGGGC10400.068.234623
ATCCGTA17000.066.972551
ATAGCGG3900.066.34811
AAGAGGG68950.066.11482
CGTACAC16400.065.9090654
GTAGGGA12500.065.794443
AGATAGG11400.065.617951
GAATAGG12550.065.603161
TAGGGAT12400.065.567044
GCATAGG5100.065.4965741
TAAGGGA17600.065.153593
AGGGCAT16450.064.565985