FastQCFastQC Report
Mon 27 Feb 2023
SRR3128326.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128326.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2212322
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314001.4193232269081988No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG37880.1712228147620464No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC35990.16267975457460532No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG32240.14572923832968257No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30670.13863262219514158No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24090.10889011635738378No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT96950.083.326561
ATAGGGC11750.072.4039463
AGGGATG30100.070.5819245
TAGGGCA10650.070.1728444
GGTAAGG12800.069.7994161
CGTAGGG4400.069.435612
GCATAGG5450.069.024231
GTAGGGC8600.068.864233
AGTAGGG34000.068.430212
ATAGCGG5250.068.0710451
TAGCGGG11200.067.5661852
TAGGGTA7100.067.524814
AGATAGG13150.067.226451
TAGGGCG3150.067.146524
ATAGGGA20250.067.08023
AGACGGG15650.066.974912
AAGGGTA15200.066.793124
AAGGGAT37150.066.676594
GTAGGGT6450.066.31373
ATGAGGG32150.066.227562
Produced by FastQC (version 0.11.5)