FastQCFastQC Report
Mon 27 Feb 2023
SRR3128325.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128325.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1929730
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284571.4746622584506641No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG31490.16318345053453073No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC31370.1625616018821286No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28170.1459789711514046No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG25680.13307561161406No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC20150.10441875288252761No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT87550.084.508471
ATAGCGG4050.075.537421
CGTAGGG4850.072.759232
TAGGGCA9350.072.463534
AGATAGG13450.072.435571
TAGACGG2100.071.719051
GGTAAGG12050.070.696081
GTAGGGC7100.070.245123
ATAGGGC10550.070.018983
GATAGGG31950.069.2142642
AGGGATG27250.068.9904865
AGTAAGG11250.068.611221
AGTAGGG32100.068.597682
ATAGGGA19100.068.236233
TAGCGGG10450.067.5372852
AAGGGAT32800.066.990264
TAGGGAT13150.066.908994
GTAGGGA13100.066.80523
TAGGGTA6450.065.652524
TAGAGGG32600.065.3806462
Produced by FastQC (version 0.11.5)