FastQCFastQC Report
Mon 27 Feb 2023
SRR3128324.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128324.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2045529
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249351.219000072841793No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCACTTCCG42390.20723245673857474No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG40910.1999971645476549No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC40750.1992149707972852No Hit
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG40220.19662395399918553No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC40180.19642840556159313No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA30100.14715019928830145No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28020.13698168053349524No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT79800.086.256671
TCCGTAC21550.075.021272
CCGTACA21400.073.5705953
GAATAGG12650.072.1543351
AGTAGGG31700.072.05272
TAGGGCA9400.071.995924
CGTAGGG4050.071.946542
ATCCGTA22950.070.9323961
CGTACAC22050.070.5492864
GGTAAGG12150.070.476821
TAGGGTA6050.069.913394
ACGGGAT7100.069.5031054
AGGGATG26350.069.381265
ATAGAGG14150.069.160491
AGACGGG13250.068.8111952
GAGGGTA10900.068.555754
AGTAAGG13150.068.3374561
TAAGGGA17950.068.335963
GTAGGGC8500.067.4553
AGAGGGC26250.067.318093