FastQCFastQC Report
Mon 27 Feb 2023
SRR3128323.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128323.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2280070
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297841.3062756845184578No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC45910.2013534672181117No Hit
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG43670.19152920743661378No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC43410.19038889156911848No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG42660.18709951887442053No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31030.136092312955304No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCACTTCCG23720.10403189375764779No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT97300.086.503071
ACGGGAT8150.073.816454
AGGGATG30050.073.5097055
TAAGGGA19300.071.839873
CTACGAC12050.071.376515
AGGGTAC9750.071.342515
GAGGGTA11950.071.188774
GAATAGG12800.069.796711
TAGGGCA11250.068.93384
ATAGGGC10850.068.876043
GAGGGAT27850.068.855044
AGTAAGG15750.068.6655041
TCCGCTA12400.068.635411
AGACGGG15000.068.620462
AGGGCAT19000.068.520015
ATAGGGA20100.068.279073
AGAGGGC31150.067.294193
AAGAGGG76100.067.258012
CGAGGGA14900.067.188373
AGTAGGG36650.066.941792
Produced by FastQC (version 0.11.5)