FastQCFastQC Report
Mon 27 Feb 2023
SRR3128322.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128322.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1982149
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266521.344601238352919No Hit
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG38090.19216517022685983No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37100.18717059111096088No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC37030.18681743905226095No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG36290.18308411728886173No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27430.13838515671627108No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85350.084.809811
AGTAAGG13550.076.761991
TAGGGCA9250.073.758124
ATAGCGG4600.073.666141
ATAGAGG12850.071.420811
GAATAGG11250.071.119671
AGAGGGC27800.070.7481463
GAGGGTA9750.070.4582444
TAGGGTA7500.070.2652054
TAAGGGA17400.070.0380553
TAGAGGG36350.069.8993152
ACGGGAT5700.069.340664
AGGGATG28550.068.813145
AGGGAAT18700.068.364175
GTACGGG3100.068.3021552
AGTAGGG33000.068.2975542
AGGGCAT16600.068.2354665
GCATAGG4900.068.195471
ATAAGGG29050.068.189812
GTAGGGA16250.068.0452963
Produced by FastQC (version 0.11.5)