FastQCFastQC Report
Mon 27 Feb 2023
SRR3128321.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128321.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2084746
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267331.282314488191847No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG36100.1731625819164541No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA35630.17090811062834513No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC33090.15872437217771374TruSeq Adapter, Index 27 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG30690.14721217836609352No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGAGTTAC28920.13872193543002362No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22270.10682356507699259No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT82500.086.058491
CCGTACA15850.074.4243
TCCGTAC16500.074.055622
CGTACAC15800.073.767184
CGTAGGG5450.072.4354862
ATAGGGC12100.072.2430653
AGTAGGG34650.069.850992
TAGGGCA11050.069.750954
ACGGGTA3100.069.737244
ATCCGTA17900.068.8662951
AGGGCAT17550.068.821585
ATAGGGA19550.068.512023
AGGGTAC11200.068.397185
TAGAGGG38150.067.877472
TAGGGTA6550.066.728334
GGTAAGG12200.066.718261
TAGCGGG12050.066.302592
ATAGAGG14100.066.070031
GTAGGGC9250.066.049613
TAGGGAT13950.065.6944964
Produced by FastQC (version 0.11.5)