FastQCFastQC Report
Mon 27 Feb 2023
SRR3128320.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128320.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2315031
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT317911.3732429500944048No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG41200.17796737927051515No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC34650.14967402164377064TruSeq Adapter, Index 27 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG33330.14397215415257938No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA24600.1062620759722008No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23670.10224485114886152No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT96850.085.939651
ATAGGGC15250.072.735483
TAGGGCA13000.071.585654
AGGGCAT22200.069.654175
GGTAAGG12650.069.142071
ATAACGG2250.068.96861
GTAGGGC10100.068.8722843
TAGAGGG42850.068.663772
AGAGGGC33150.068.480633
GAGGGAT29400.067.783294
AGTAGGG40500.067.541722
AGATAGG13700.067.275271
ATAGGGA20700.067.20873
TAAGGGA18300.067.033763
AAGGGCG10300.066.622334
GAATAGG14050.066.603451
AAGAGGG84250.066.554082
ATAAGGG33000.065.800962
AGGGATG30850.065.206785
CCGTACA11850.065.0473563
Produced by FastQC (version 0.11.5)