FastQCFastQC Report
Mon 27 Feb 2023
SRR3128319.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128319.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2023743
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281471.3908386588613277No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG32810.16212532915493716No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC28590.14127287901675262TruSeq Adapter, Index 27 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG27820.13746804806736823No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22300.11019185736528798No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86450.085.487651
ACGGGTA2600.076.008474
TAGGGCA11700.074.399824
GAGGGAT27050.073.058054
ATAGGGC11900.071.56783
GTAGGGC10300.070.807713
AGTAGGG34300.070.7850652
TAGAGGG34150.070.5448462
AGGGCAT19200.069.033755
GGTAAGG10900.068.665621
AGAGGGC28050.068.440523
AGGGATG30100.068.394515
ATAGGGA17250.066.8287053
TAAGGGA18100.066.809863
AAGGGCG9650.066.800444
AGATAGG11000.066.329661
ACGGGAT6600.066.301764
ATAGAGG14650.066.19091
AAGGGCA32750.065.945854
CGAGGGA10500.065.874013
Produced by FastQC (version 0.11.5)