FastQCFastQC Report
Mon 27 Feb 2023
SRR3128318.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128318.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2508151
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355811.418614748474075No Hit
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC58030.23136565541707815Illumina Single End Adapter 1 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT45090.17977386528960976No Hit
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG41540.16562001251120845No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCGCTAGT39890.15904146121983884No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36540.14568500859796718No Hit
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG34400.1371528269230999No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA30170.12028781361249781No Hit
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT25120.10015345966012414No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT116200.086.0857541
ATAGCGG5900.081.34341
CCGTACA17100.075.578743
AGGGATG39350.075.1223145
AGTACGG3150.074.685041
TCCGTAC18400.072.7930762
GTACGGG6400.071.962982
ATAACGG2400.070.577361
TATAGGG20800.069.816522
AATGCGG6550.069.679431
CGTACAC18450.069.539144
TAGCGGG14550.069.44462
ATAGGGA22600.069.246663
ATCCGTA19650.069.200531
GGTAAGG13750.069.123031
AGTAGGG43500.069.035852
TAAGGGA21800.068.1229863
ATAGGGC14250.067.9384163
ATAGAGG17800.067.934011
AAGGGAT42850.067.4508364