FastQCFastQC Report
Mon 27 Feb 2023
SRR3128317.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128317.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2791447
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT426791.5289203054903067No Hit
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC61020.2185963050704527Illumina Single End Adapter 1 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT50330.18030075441160087No Hit
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG46950.16819233895538765No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC41080.14716381862166827No Hit
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG37880.13570023002406995No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT136650.085.655321
TAGGGCA14000.076.544234
AGTACGG4050.074.312871
AGGGATG44100.074.283455
GAATAGG16350.073.63111
AGTAGGG46850.072.7347262
GAGGGAT37350.071.0994
ATAGGGA23050.070.55353
ATAGGGC17700.070.104213
ATAGCGG5250.069.867371
GGTAAGG16650.069.762461
TAAGGGA24600.069.7382663
ATAACGG2700.069.668321
AGATAGG14100.069.371861
GTAGGGA21650.068.820023
ATGAGGG45850.068.580432
AATGCGG6050.068.401631
ATAAGGG36100.068.093852
CCGTACA12450.067.954253
GTACGGG7750.067.318542
Produced by FastQC (version 0.11.5)