FastQCFastQC Report
Mon 27 Feb 2023
SRR3128316.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128316.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2423913
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT375281.5482403865155225No Hit
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC54940.2266583000297453Illumina Single End Adapter 1 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT44420.1832574023902673No Hit
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG37700.1555336350768365No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35190.14517847794042113No Hit
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG32120.13251300686121986No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT115000.085.615691
ATAGCGG4250.077.5543751
AGGGATG39250.074.1225055
CGTAGGG6550.072.58432
GAATAGG13050.072.524171
TAGGGTA7300.072.220054
GTACGGG5300.071.940222
TAGGGCA11500.071.63134
AGTAGGG39300.071.506312
ATAGGGC13500.071.47973
ATAACGG2550.070.168241
ATAGGGA23100.069.6913
AGTAAGG13950.069.532881
GAGGGAT32250.069.184894
GTAGGGA17150.069.167013
GGTAAGG13350.069.130871
TAAGGGA21350.069.009523
TATAGGG19950.068.661412
TAGAGGG45550.068.618672
TAGCGGG12450.068.433992
Produced by FastQC (version 0.11.5)