FastQCFastQC Report
Mon 27 Feb 2023
SRR3128314.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128314.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences945841
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139521.475089364914399No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG54930.5807530018258883No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC54890.5803300977648463No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGAATCACG51830.5479779370951354No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC47900.5064276130977617No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG44330.4686834256497656No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA18040.19072973152992945No Hit
CGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT17760.18776940310263565No Hit
AGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT16360.17296776096616662No Hit
AAAGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTT15970.1688444463710074No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATG14890.15742603672287414No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGAA13620.14399883278479153No Hit
ACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG13610.14389310676953104No Hit
AGAGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTT13060.1380781759302039No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC11770.12443951996160031No Hit
ACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT11560.12221927364112996No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCGAATCACGTC11470.12126773950378551No Hit
GGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT11430.12084483544274355No Hit
GGAACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTT9870.10435157706210664No Hit
GGAGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTT9750.10308286487898072No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT54000.089.064881
TAGGGCG950.084.1031344
GCTACGA11350.083.231384
AGGGATG19950.081.982895
CGCTACG11550.081.790143
GAGGGTA4700.080.997944
GTACGAT353.864625E-880.569388
CGGGATG4500.080.420195
CGAAGGG7750.080.049582
TCCGCTA11950.079.077481
TAAGGGA9050.078.937233
CTACGAC12100.078.460825
CGAGGGA5950.078.19973
TATAGGG7000.077.883742
GGATGGC16350.077.612727
AGAGGGC10750.077.384093
AGGGAAT13450.077.2248155
AGGGCAT7400.076.84945
ATAGGGA10850.076.670873
CGTAGGG2950.076.472642
Produced by FastQC (version 0.11.5)