FastQCFastQC Report
Mon 27 Feb 2023
SRR3128313.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128313.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences807447
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120131.4877756682481946No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG45050.5579313564853172No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC43600.5399735214819054No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC39680.4914254434037157No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG36830.4561290090866645No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGAATCACG26790.3317864825802808No Hit
CGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT14480.17933065575821075No Hit
AAAGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTT13800.1709090503773003No Hit
AGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT13400.16595516485911768No Hit
AGAGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTT11030.13660339316388567No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGAA10950.13561261606024916No Hit
ACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG10250.1269433164034296No Hit
GGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT9880.12236097229911065No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA9430.1167878510911552No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC9130.11307243695251824No Hit
ACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT9120.11294858981456368No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG8950.11084318846933607No Hit
GGAGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTT8700.10774701002047193No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGG156.8705005E-494.10641
CGTTTTT47700.089.470121
GATTAGG1550.088.035011
ATAGCGG650.086.867431
CGAGGGA3800.086.650083
AGGGATG18850.083.5291755
CGCTACG8150.082.534013
GCTACGA8100.082.462754
CGAAGGG7100.081.489432
GGATGGC15350.080.830157
GGGTACG1050.080.57276
TAAGGGA8550.080.323253
GTACGGG1350.080.139862
CGTAGGG2000.079.9656452
AAGGGAT16350.079.116944
AATGCGG1250.079.049371
TACGAGG1250.079.049371
GAGGGTA3900.078.397694
AGGGTAG3250.078.0935445
CAATCGG1150.077.740071
Produced by FastQC (version 0.11.5)